We used the SCENITH kit and this allowed us to demonstrate the metabolic dichotomy between gamma delta T cell subsets in the thymus, periphery but also in the tumor microenvironment *1. The kit is easy to use and very useful to study the metabolic profile of cells in rare cell populations. We obtained very rapidly and in a simple way a large amount of information about metabolism like glycolytic capacity, glucose/mitochondria dependency, fatty acid oxidation capacity. SCENITH is the best technology to analyze and deepen our knowledge in the metabolism field, specially for rare but important immune cells types